Yes, but only the homebrew games DScraft or DSome. No official Minecraft.
However, this game was published on a standard 3DS cartridge. It will not boot or run on an original Nintendo DS or Nintendo DS Lite. The Rise of Minecraft DS Homebrew ROMs nintendo ds minecraft rom
Ensure your flashcart's custom firmware (like YSMenu or Wood R4) is properly installed on the MicroSD root. Yes, but only the homebrew games DScraft or DSome