: The acceptable length of the final PCR product (e.g., 150-300 ). Thermodynamic and Physical Thresholds
SEQUENCE_ID: MY_SEQUENCE SEQUENCE: atgccatgccatgccatgccatgccatgcc PRIMER_OPT_TM: 65 PRIMER_MIN_TM: 60 PRIMER_MAX_TM: 72 PRIMER_OPT_SIZE: 20 PRIMER_MIN_SIZE: 18 PRIMER_MAX_SIZE: 24 PRIMER_MIN_GC: 40 PRIMER_MAX_GC: 60 PRIMER_PAIR_SPECIFICITY: high primer3 0.4.0
Let us know in the comments if you need a deep dive into those specific parameters! : The acceptable length of the final PCR product (e
: The acceptable length of the final PCR product (e.g., 150-300 ). Thermodynamic and Physical Thresholds
SEQUENCE_ID: MY_SEQUENCE SEQUENCE: atgccatgccatgccatgccatgccatgcc PRIMER_OPT_TM: 65 PRIMER_MIN_TM: 60 PRIMER_MAX_TM: 72 PRIMER_OPT_SIZE: 20 PRIMER_MIN_SIZE: 18 PRIMER_MAX_SIZE: 24 PRIMER_MIN_GC: 40 PRIMER_MAX_GC: 60 PRIMER_PAIR_SPECIFICITY: high
Let us know in the comments if you need a deep dive into those specific parameters!