: High protein concentrations can bind LPS through hydrophobic or electrostatic interactions, forming biologically inactive complexes
Traditional endotoxin assay interference—caused by pH extremes, glucans, or other matrix components—is typically detectable at the time of testing and can often be resolved through dilution. LER, by contrast, presents three uniquely challenging characteristics: pda technical report 82
Perhaps most importantly, TR 82 discusses methods to "unmask" endotoxins, such as using specific sample treatments or alternative detection methods like Recombinant Factor C (rFC). Why It Matters for Your Facility : High protein concentrations can bind LPS through